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Image Search Results
Journal: Journal of visualized experiments : JoVE
Article Title: 4D Light-sheet Imaging of Zebrafish Cardiac Contraction
doi: 10.3791/66263
Figure Lengend Snippet: Table of Materials
Article Snippet: RESOURCE SOURCE/Reference IDENTIFIER Animal models Tg(myl7:nucGFP) transgenic zebrafish Burns Lab in Boston Children’s Hospital ZDB-TGCONSTRCT-070117-49 Software and algorithms MATLAB The MathWorks Inc. R2023a LabVIEW National Instruments Corporation 2017 SP1 HCImage Live Hamamatsu Photonics 4.6.1.2 Python The Python Software Foundation 3.9.0 Fiji-ImageJ Schneider et al. 18 1.54f 3DeeCellTracker Chentao Wen et al. 15 v0.5.2 Unity Unity Software Inc. 2020.3.2f1 Amira Thermo Fisher Scientific 2021.2 3D Slicer Andriy Fedorov et al. 17 5.2.1 ITK SNAP Paul A Yushkevich et al. 16 4 Light-sheet system Cylindrical lens Thorlabs ACY254-050-A 4X Illumination objective Nikon MRH00045 20X Detection objective Olympus 1-U2M585 sCMOS camera Hamamatsu C13440-20CU Motorized XYZ stage Thorlabs PT3/M-Z8 Two-axis tilt stage Thorlabs GN2/M Rotation stepper motor Pololu 1474 Fluorescent beads Spherotech FP-0556-2 473nm DPSS Laser Laserglow R471003GX 532nm DPSS laser Laserglow R531003FX Microinjector and vacuum pump Microinjector WPI PV850 Vacuum pump Welch 2522B-01 Pre-Pulled Glass Pipettes WPI TIP10LT Capillary tip for gel loading Bio-Rad 2239912 Virtual reality hardware VR headset Meta Quest 2 30mg/L PTU solution PTU Sigma-Aldrich P7629 1X E3 working solution - - 1% Agarose Low-melt agarose Thermo Fisher 16520050 Deionized water - -
Techniques: Transgenic Assay, Software